Cytochalasin b glucose uptake

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WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … WebEnter the email address you signed up with and we'll email you a reset link.

Cytochalasin B - an overview ScienceDirect Topics

WebMay 9, 2024 · We artificially blocked the RBC GLUT1 transporter using either one of two routinely used GLUT1 inhibitors: cytochalasin B (CCB) and forskolin. These inhibitors bind GLUT1 in the transmembrane region, effectively blocking RBC D-glucose uptake (Guizouarn and Allegrini 2024 ). WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. … port paye in english

Cytochalasin A and B - Journal of Biological Chemistry

WebVisfatin stimuli also enhanced intracellular NAD levels, and treatment with FK866, which is a specific inhibitor of nicotinamide phosphoribosyltransferase (Nampt), significantly inhibited visfatin-induced NAD synthesis and glucose uptake. Visfatin treatment increased glucose transporter-1 (GLUT-1) protein expression in isolated cellular ... WebMay 13, 2024 · Impairment of cellular glucose uptake due to compromised insulin ... we report the near atomic resolution cryo-EM structures of GLUT4 bound to a small … Web2.2 Assaying Insulin-Stimulated Glucose Uptake 1. Serum-free media: DMEM (with 4.5 g/L glucose, l -glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin (BSA). 2. 2-Deoxyglucose (Sigma) stock: 100 m M (16.4 mg/mL in double-distilled water). Store at 2°–4°C up to 2 wk. 3. iron on inside patches for jeans

Comparison of Glucose Uptake Assay Methods

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WebRCME cells exhibited stereospecific hexose uptake inhibited by cytochalasin B. Pretreatment of the RCME cells with potassium cyanide or 2,4-dinitrophenol inhibited 2-deoxyglucose uptake but not 3-O-methylglucose transport. A major proportion (30–60%) of the 2-deoxyglucose present in the RCME cells was not phosphorylated. port pavilion on broadway pier san diegoWebApr 23, 2015 · Moreover, cytochalasin B, a nonspecific glucose transport inhibitor, also strongly inhibited the uptake of 14 C-glucose, 14 C-2DG and 2DG-IR in neuronal and astrocytic cultures . port paye finlande

"WebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 … " - Cytochalasin b glucose uptake

Cytochalasin b glucose uptake

In vivo imaging of the dynamics of glucose uptake in the …

WebFeb 27, 2024 · The present study was conducted to explore the mechanism of nano-Zn absorption and its influence on lipid metabolism in the intestine of yellow catfish Pelteobagrus fulvidraco. Compared to ZnSO4, dietary nano-Zn addition increased the triglyceride (TG) content, enzymatic activities of malic enzyme (ME) and fatty acid … WebMay 13, 2024 · A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo. ... To examine the effect of cytochalasin B ...

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WebNov 11, 2008 · Cytochalasin B (10 μmol/L) and phloretin (20 μmol/L) inhibited glucose uptake 85 ± 1% and 84 ± 18%, respectively. In contrast, the selective GLUT4 inhibitor … WebHepG2 cells treated with agents affecting glucose uptake/metabolism (phloretin, quercetin, quercetin-3′-sulfate, NaF, 3-bromopyruvate, NaN3, oligomycin A, ochratoxin A, cytochalasin B, and anti-GLUT1 antibody) showed dose-dependent changes in glucose and ATP levels without total protein (cell) loss. Finally, we performed flow cytometric ...

WebA novel phosphoinositolglycan-peptide (PIG-P) from the yeast Saccharomyces cerevisiae potently mimicks insulin action on glucose transport and metabolism in rat muscle and adipose tissue. The aim of the present study was to elucidate the cellular WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ...

Web2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin … WebJun 18, 2024 · The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases.

WebCytochalasin B, which is often used as an inhibitor of actin-based motility systems, is also a very potent competitive inhibitor of Đ-glucose uptake into mammalian cells. When red blood cell ghosts (yes it's a real term, these are RBCs emptied of their cytoplasm) are incubated when 'H-cytochalasin and then irradiated with ultraviolet light, the

WebA mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy- d -glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at … iron on interfacing hobbycraftWebMay 10, 1972 · A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is … iron on interfacing walmartWebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range. iron on interfacing wholesaleWebIn Fig. 4 (right side), we summarize the re- sults of 4 -6 independent experiments, comparing the insulin-dependent net change in cytochalasin B binding sites with the net change in GLUT4 glucose ... iron on jean patches targetWebJul 1, 1974 · Cytochalasin B in this concentration suppresses multiplication rates in a nutrient medium consisting of 2 % proteose peptone, but multiplication is unaffected if … iron on jersey numbersWebNov 11, 2008 · Non-carrier-mediated uptake was determined in parallel wells containing 10 μmol/L cytochalasin B. Protein concentration was measured by the modified Bradford protein assay. Cell-associated radioactivity was determined by scintillation counting. The mean counts/min were expressed as pmol/min/mg protein from determinations done in … iron on jersey nameWebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ... port paye tracking