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WebMar 9, 2024 · An injector for an HPLC system should provide an injection of the liquid sample within the range of 0.1-100 mL of volume with high reproducibility and under high pressure (up to 4000 psi). 4. Columns WebHigh-performance liquid chromatography is an established separation technique that is widely used for the separation of non-volatile species. Instrumental components of an HPLC are outlined including notable developments and innovations, particularly with respect to pump and column technology. WebHigh performance liquid chromatography (HPLC) facilitates the rapid, quantitative separation of compounds from each other and from the other constituents of complex mixtures or matrices. Used in tandem, the two techniques (usually referred to as LC-MS) provide a unique capability for rapid, cost-effective and quantitative measurements of … fluid diet for weight loss