Sprr2f-cre

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August undefined, 2024

Web9 Nov 2024 · Generation of Sprr2f promoter Cre with ZsGreen1 reporter mice. The Tg(Sprr2f-cre)1Dcas transgenic (Tg) mouse in an FVB background was obtained from the Jackson Laboratory (Sacramento, CA).Generation of this mouse, which has a 5.5-kb promotor sequence from the Sprr2f gene upstream of Cre, has been previously described …

Sprr2f protects against renal injury by decreasing the level of ...

Webuterine epithelial cells using Sprr2f-cre mice (Sprr2fcre+Mig-6f/f). Female mutant mice develop endometrial hyperplasia due to aberrant phosphorylation of STAT3 and … WebNumber: Citing Articles: 1: Anuchina AA, Zaynitdinova MI, Demchenko AG, Evtushenko NA, Lavrov AV, Smirnikhina SA. Bridging Gaps in HDR Improvement: The Role of MAD2L2 ... crispy thin pizza dough recipe

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http://www.informatics.jax.org/allele/genoview/MGI:4438263 Web23 Jul 2024 · BAC-Sprr2f-Cre is a BAC-transgenic line with Cre inserted into the Sprr2f locus, which is expressed exclusively in the endometrial epithelial cells that give rise to all … WebIdentification of Sprr2f as an estrogen-dependent gene that is specifically expressed in the uterus. (A) Digital northern representation, per Affymetrix 430 2.0 array data, of Sprr2f expression in... man diagnose support

Tg(Sprr2f-cre)1Dcas Transgene Detail MGI Mouse …

037052 - BAC-Sprr2f-Cre Strain Details - The Jackson …

Web26 Jan 2024 · Anti-mullerian hormone receptor type 2 ( Amhr2 )-Cre mice are mainly used to target genes in stromal and myometrial compartments of the uterus and oviduct as well as of the ovary. 10 Temporally, Cre action starts from midgestational embryo development (embryonic day 12.5) under the control of the Amhr2 promoter in Amhr2 -Cre mice. WebIn summary, Sprr2f-Cre was expressed in most Müllerian epithelial cells of the uterus, oviduct and cervix. Cre-mediated activity was also evident in the urothelium of the ... mandeville la to montgomery alWebSince BAC-Sprr2f-Cre leads to subtotal mosaic Cre-mediated recombination with only 50% efficiency even in aged mice (43), this result further confirmed a potent growth advantage … crispy tofu sisig

"WebSprr2f gene is expressed only in the uterus, and therein only within endometrial epithelium, the presumptive cell of origin for all ECs. In these prior studies, a 5.5-kb Sprr2f promoter fragment was fused to Cre to generate a “minigene” transgenic driver line Sprr2f-Cre, a.k.a. Tg(Sprr2f-cre)1Dcas (22). This allele drives Cre-mediated re- " - Sprr2f-cre

Sprr2f-cre

JCI Insight - A PoleP286R mouse model of endometrial cancer ...

WebSprr2fGene Detail Summary Symbol Sprr2f Name small proline-rich protein 2F Feature Type protein coding gene IDs MGI:1330349 NCBI Gene: 20760 Alliance gene page Transcription … WebCre; PtenL/L or BAC-Sprr2f-Cre; Foxa2L/L, PtenL/L mice. The uteri were washed in PBS, cut into small pieces (2-3 mm) using sterile scalpels and digested in Collagenase V/Dispase II enzymatic solution (0.4 mg/ml Collagenase V and 1.25 …

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Web26 Jan 2024 · Anti-mullerian hormone receptor type 2 (Amhr2)-Cre mice are mainly used to target genes in stromal and myometrial compartments of the uterus and oviduct as well … WebMETHOD: To further understand the role of Mig-6 and P4 in controlling uterine proliferation, we developed a Sprr2f-cre driven mouse model where Mig-6 is specifically ablated only in the epithelial cells of the uterus (Sprr2f RESULTS: Sprr2f CONCLUSIONS: These data suggest that endometrial epithelial cell proliferation is regulated by P4 mediated Mig-6 …

http://www.cancerindex.org/geneweb/ERRFI1.htm Web25 Sep 2013 · Sprr2f-Cre; Lkb1L/L endometrial Lkb1-knockout mice were bred and generated as previously described [10]. Breeding of LSL-KrasG12V; Lkb1L/L mice and Adeno-Cre nasal instillation to effect bronchial Cre-mediated recombination was conducted as previously described [2]. Mice were treated with Adeno-Cre at 8 weeks of age and …

Web037052 B6(FVB)-Tg(Sprr2f-cre)2Dcas/J BAC-Sprr2f-Cre transgenic mice express Cre recombinase under the control of the Sprr2f promoter. They may be useful for driving … Web24 Feb 2024 · Cre-ERT2 and Pten knockout mice were obtained from Jackson Laboratory. Sprr2f-Cre mice were provided by Dr. Leone. Cyclin D1 Flox/WT mice were generated in …

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WebStock No: 013148 Protocol 22392: Standard PCR Assay - Generic Cre Version 1. 3 Notes This assay will NOT distinguish hemizygous from homozygous transgenic animals. The genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). crispy tofu dinnerWeb15 Jun 2024 · BAC-Sprr2f-Cre was bred to mice harboring a floxed Foxa2 fl allele, where LoxP sites flank exon 3. This exon encodes almost the entire protein, including the DNA … crispy tofu dipping sauceWebpremature death ( J:158190 ) • females display a marked increase in mortality; females begin to die at 17 weeks of age and all females are dead by 30 weeks. • death in most … crispy tomato hoagieWeb5 Oct 2024 · The Tg(Sprr2f-cre)1Dcas mouse was crossed with a C57/BL6 Ai6-ZsGreen1reporter mouse pur-chased from the Jackson Laboratory (stock no. 007906) to generate a mouse in which cells that express Sprr2f during development were per-manentlylabeledﬂuorescently. Generation of Sprr2f-KO mice. C57/BL6 mice harboring a … crispy tofu recipeWebMutation details: A BAC derived 5.5 kb fragment of the Sprr2f promoter was cloned into a vector containing a cre coding sequence. Cre is expressed exclusively in endometrial … crispy tofu recipes chineseWeb1 Oct 2024 · Sprr2f-Cre;Pten fl/fl mice develop in situ carcinoma with 100% penetrance at an early age. In situ carcinomas are characterized by neoplastic expansion of the glandular … mandia cottageWeb18 Sep 2024 · Mitogen-inducible gene 6 ( Mig-6) is a critical mediator of progesterone receptor (PGR) action in the uterus. In order to study the function of Mig-6 in P4 resistance, we generated a mouse model in... crispy treat 100mg delta 9